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Related Experiment Video

Updated: May 14, 2026

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

OsORC3 is required for lateral root development in rice.

Xinai Chen¹, Jing Shi, Xi Hao

¹The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China.

The Plant Journal : for Cell and Molecular Biology

|January 26, 2013

Summary

The origin recognition complex subunit 3 (OsORC3) is essential for rice lateral root development. OsORC3 deficiency in rice causes defects in lateral root emergence and plant growth.

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Published on: October 6, 2020

Area of Science:

Plant molecular biology
Genetics
Developmental biology

Background:

The origin recognition complex (ORC) is vital for DNA replication and chromosome regulation in eukaryotes.
While ORC functions are known in yeast and animals, their roles in plants are largely unexplored.
Understanding plant ORC function is crucial for crop improvement.

Purpose of the Study:

To clone and functionally characterize Oryza sativa Origin Recognition Complex subunit 3 (OsORC3) in rice.
To investigate the role of OsORC3 in plant development, particularly lateral root formation.
To elucidate the molecular mechanisms underlying OsORC3's function in rice.

Main Methods:

Map-based cloning was used to identify the gene responsible for the mutant phenotype.
Functional characterization involved creating and analyzing an orc3 mutant and OsORC3 knockdown plants.
Expression analysis and cellular assays (CYCB1;1::GUS, methylene blue staining) were performed.

Main Results:

A G→A mutation in the 9th exon of OsORC3 caused a temperature-dependent defect in lateral root development.
OsORC3 is highly expressed in actively proliferating tissues, including root tips and primordia.
OsORC3 knockdown resulted in a lack of lateral roots, dwarfism, increased cell death in root meristems, and arrested lateral root primordia development.

Conclusions:

OsORC3 plays a critical role in the emergence and development of lateral roots in rice.
OsORC3 is essential for maintaining cell viability and proliferation in the root meristem.
This study highlights OsORC3 as a key regulator of plant architecture and development.