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Reticulocyte count in red-blood-cell units stored in AS-1.

A Urbina¹, F Palomino

¹School of Medicine and Health Sciences, Basic Science Department, Physiology Unit, Universidad del Rosario, Bogotá, DC, Colombia. adriana.urbina@urosario.edu.co

|January 26, 2013

Summary

This summary is machine-generated.

Reticulocyte count significantly decreases in red blood cell units stored in AS-1, suggesting maturation during storage. This study investigated reticulocyte behavior in additive solutions, unlike previous whole blood studies.

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Area of Science:

Blood banking and transfusion medicine.
Hematology and cell biology.

Background:

Previous studies on whole blood stored in CPDA-1 suggested arrested reticulocyte maturation.
Reticulocyte behavior in red blood cell units stored in additive solutions (AS-1) remained unstudied.

Purpose of the Study:

To determine reticulocyte count and subtypes in red blood cell units stored in AS-1.
To investigate reticulocyte maturation during refrigerated storage in additive solutions.

Main Methods:

Flow cytometry with thiazole orange was used to analyze reticulocyte percentage and subtypes.
Six red blood cell units stored in AS-1 were examined.

Main Results:

Reticulocyte count decreased from 26.8 ± 4.6 × 10(9)/l at week 0.5 to 8.2 ± 2.9 × 10(9)/l at week 6.
High-fluorescence reticulocytes decreased significantly by week 2, while low-fluorescence reticulocytes decreased by week 4.
Total hemolysis was low at 0.19 ± 0.08%.

Conclusions:

A significant decrease in reticulocytes occurs during storage in AS-1.
Reticulocyte maturation appears to occur during storage, as subtype changes exceed levels explainable by hemolysis alone.