Related Experiment Video
Updated: May 14, 2026
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
In vitro effect of paediatric liquid medicines on deciduous enamel exposed to biofilm
Daniela Novaes Soares1, Andréa Gonçalves Antonio, Viviane Santos da Silva Pierro
1Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Objective:
This study aimed to assess the in vitro effects of paediatric liquid medicines on deciduous enamel exposed to biofilms.
Methods:
Fragments (n = 25) of first primary molars were covered by nail varnish, leaving a 22 mm(2) exposure area. Specimens were fixed in polystyrene plates containing BHI broth media. Pooled human saliva was added to form a mature biofilm on fragments over a 10-day period in microaerophilic conditions. Specimens were divided into groups (n = 5 per group) and treated (50 μL) daily for 1 min over 1 week as follows: G1 = 10% sucrose solution (positive control); G2 = Dimetapp Elixir® (antihistamine); G3 = Claritin® (antihistamine); and G4 = Klaricid® (antibiotic). Five other fragments, without treatment and inoculum represented the blank controls. The covered area for each specimen represented the negative control. Cross-sectional hardness of the enamel was used as a demineralization indicator.
Results:
All treatment groups showed hardness loss compared to the corresponding negative controls (p < 0.05). Among the treatment groups, G2 exhibited the greatest demineralization pattern (p < 0.05) followed by G3, G1 and G4.
Conclusion:
All medicines caused deciduous enamel demineralization in the presence of biofilm. The greatest hardness loss was observed after treatment with Dimetapp Elixir®.
