Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Adaptability of Cytoskeletal Filaments

Adaptability of Cytoskeletal Filaments

The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...

Mechanism of Filopodia Formation

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

Pinching-off of Coated Vesicles

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

Microtubule Associated Motor Proteins

Microtubule Associated Motor Proteins

Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A comparative analysis of vortioxetine vs. escitalopram on the clinical profile of patients with major depressive disorder.

Journal of family medicine and primary care·2026

Same author

Discovery of a small molecule TLR3 agonist adjuvant.

Nature communications·2025

Same author

Incorporating meningeal immunity into vaccine development.

Nature communications·2025

Same author

Adjuvantation of a SARS-CoV-2 mRNA vaccine with controlled tissue-specific expression of an mRNA encoding IL-12p70.

Science translational medicine·2024

Same author

Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance.

The Journal of allergy and clinical immunology·2023

Same author

Publisher Correction: Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice.

NPJ vaccines·2023

Search research articles

Related Experiment Video

Updated: May 14, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Dynamin: expanding its scope to the cytoskeleton.

Manisha Menon¹, Dorothy A Schafer

¹Department of Biology, University of Virginia, Charlottesville, VA, USA.

International Review of Cell and Molecular Biology

|January 29, 2013

Summary

The large GTPase dynamin, crucial for vesicle formation, also influences the cytoskeleton. Emerging evidence suggests dynamin affects actin filaments and microtubules through novel, non-membrane-remodeling mechanisms.

More Related Videos

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

Published on: January 24, 2025

Related Experiment Videos

Last Updated: May 14, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

Published on: January 24, 2025

Area of Science:

Cell Biology
Molecular Biology

Background:

Dynamin is a large GTPase primarily known for its role in vesicle formation, particularly clathrin-coated endocytic vesicles.
Dynamin's functions extend to secretory vesicle formation, endosomal trafficking, and membrane remodeling during exocytosis and fusion.
Recent research indicates dynamin interacts with the cytoskeleton, influencing cellular processes beyond membrane dynamics.

Purpose of the Study:

To explore the emerging role of dynamin in cytoskeletal regulation.
To discuss evidence supporting dynamin's influence on actin filaments and microtubules.
To consider potential mechanisms by which dynamin interacts with the cytoskeleton.

Main Methods:

Review of recent scientific literature and evidence.
Analysis of studies investigating dynamin's interactions with cytoskeletal components.
Hypothetical modeling of dynamin-cytoskeleton interplay.

Main Results:

Dynamin exhibits functions beyond membrane remodeling, impacting cytoskeletal networks.
Evidence suggests dynamin influences both actin filaments and microtubules.
These effects appear to be independent of dynamin's canonical membrane-binding and scission activities.

Conclusions:

Dynamin's role in cellular processes is more diverse than previously understood, encompassing cytoskeletal regulation.
Further research is needed to elucidate the precise mechanisms of dynamin-cytoskeleton interaction.
Understanding this interaction could reveal new insights into cell shape, movement, and intracellular transport.