Related Experiment Video
Updated: May 14, 2026

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Published on: October 20, 2018
Signal amplification in immunohistochemistry: loose-jointed deformable heteropolymeric HRP conjugates vs. linear
Igor Buchwalow1, Werner Boecker, Eduard Wolf
1Institute for Hematopathology, Fangdieckstr. 75a, 22547 Hamburg, Germany. buchwalow@pathologie-hh.de
Abstract:
Improvements in reagents and protocols for immunohistochemistry have led to increased sensitivity of detection systems. A significant level of signal amplification was achieved by the chain-polymer conjugate technology utilizing enzyme-labeled inert "backbone" molecule of dextran (Dako). However, the relatively large size of the dextran molecule in aqueous phase appears to create spatial hindrance compromising the penetrative ability of the detection reagent. Novel AmpliStain™ detection systems (SDT GmbH, Baesweiler, Germany) seem to overcome these constraints offering a more compact and deformable conjugate design that facilitates agile penetration through the narrowest diffusion pathways in tissue sections. Here, we compared the level of signal amplification achievable with AmpliStain™-HRP (SDT) and EnVision™+-HRP (Dako). Our results show that the AmpliStain™-HRP systems allow higher dilutions of primary antibodies in both immunohistochemistry and ELISA. Compared with EnVision™+, anti-mouse AmpliStain™ enables at least three times more sensitive detection of mouse antibodies, whereas anti-rabbit AmpliStain™ is ten times more sensitive than anti-rabbit EnVision™+.
More Related Videos
Related Concept Videos
Immunocytochemistry and Immunohistochemistry
These...
Immunogold Electron Microscopy

