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Ultrasonic gene and drug delivery using eLiposomes.

Marjan Javadi¹, William G Pitt, Christopher M Tracy

¹Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|January 29, 2013

Summary

Ultrasound-triggered eLiposomes enhance drug and gene delivery. Folate-targeted eLiposomes with perfluoropentane emulsions effectively deliver therapeutics to the cytosol of HeLa cells.

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Area of Science:

Biotechnology
Nanomedicine
Drug Delivery Systems

Background:

eLiposomes are advanced liposomal formulations designed for targeted therapeutic delivery.
Ultrasound application triggers the phase transition of encapsulated emulsions, leading to eLiposome rupture and payload release.

Purpose of the Study:

To investigate the efficacy of folate-targeted eLiposomes containing perfluoropentane (PFC5) emulsions for drug and gene delivery.
To evaluate the influence of folate ligands, PFC5 emulsions, and ultrasonic parameters on delivery into HeLa cells.

Main Methods:

Encapsulation of model drug (calcein) or GFP plasmid within folated eLiposomes containing PFC5 emulsions.
Utilizing confocal microscopy to quantify drug delivery and gene transfection efficiency in HeLa cells.

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Applying controlled ultrasonic acoustic parameters to trigger payload release.

Main Results:

Folate ligands and internal PFC5 emulsions were crucial for effective drug and gene delivery.
Ultrasound application significantly enhanced both drug delivery and plasmid transfection rates.
Therapeutics were delivered to the cytosol, suggesting disruption of both eLiposomes and endosomes.

Conclusions:

Folate-targeted eLiposomes with PFC5 emulsions offer a promising ultrasound-triggered platform for enhanced drug and gene delivery.
The mechanism involves ultrasound-induced expansion and rupture of emulsion droplets, facilitating cytosolic delivery.
This technology holds potential for targeted intracellular delivery of therapeutics.