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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

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Related Experiment Video

Updated: May 14, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Helicase-mediated changes in RNA structure at the single-molecule level.

Sebastian L B König1, Pramodha S Liyanage, Roland K O Sigel

  • 1Institute of Inorganic Chemistry, University of Zurich, Zurich, Switzerland.

RNA Biology
|January 29, 2013
PubMed
Summary

Single-molecule spectroscopy reveals how RNA helicases interact with RNA. This review details advanced techniques for studying these crucial molecular machines and their functions in RNA processing.

Keywords:
AFMDEAD-boxFRETPIFERNA foldingRNA helicaseoptical tweezerssingle-molecule spectroscopy

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Related Experiment Videos

Last Updated: May 14, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
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Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

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Published on: February 12, 2022

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • RNA helicases are essential enzymes that modify RNA structure and function.
  • Their roles include RNA unwinding, remodeling, export, and degradation.
  • Understanding these processes is vital for cell biology.

Purpose of the Study:

  • To review the application of single-molecule spectroscopic techniques in characterizing helicase-RNA interactions.
  • To highlight recent advancements in studying these molecular mechanisms.

Main Methods:

  • Single-molecule spectroscopy techniques are presented.
  • Focus on methods that provide high-resolution insights into enzyme-substrate dynamics.

Main Results:

  • Single-molecule approaches offer unprecedented detail on helicase-RNA interplay.
  • These methods allow visualization of dynamic interactions and conformational changes.

Conclusions:

  • Single-molecule techniques are powerful tools for dissecting RNA helicase mechanisms.
  • Continued advancements promise deeper understanding of RNA processing and regulation.