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Related Experiment Video

Updated: May 14, 2026

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

Corneal endothelial regeneration and tissue engineering.

Tatsuya Mimura1, Satoru Yamagami, Shiro Amano

  • 1Department of Ophthalmology, Tokyo Women's Medical University Medical Center East, 2-1-10 Nishiogu, Arakawa-ku, Tokyo 116-8567, Japan. mimurat-tky@umin.ac.jp

Progress in Retinal and Eye Research
|January 29, 2013
PubMed
Summary

Tissue engineering using cultured human corneal endothelial cells (HCECs) shows promise for treating corneal endothelial dysfunction. Transplanted HCEC sheets effectively restore corneal clarity, offering an alternative to donor corneas.

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Human corneal endothelial cells (HCECs) possess limited proliferative capacity, creating a need for alternatives to donor corneas in endothelial keratoplasty.
  • Descemet stripping with automated endothelial keratoplasty (DSAEK) is a standard treatment but relies on donor tissue, leading to shortages.
  • Corneal endothelial deficiency necessitates innovative solutions due to limited cell regeneration.

Purpose of the Study:

  • To review corneal endothelial wound healing mechanisms and tissue engineering strategies.
  • To evaluate the efficacy of tissue-engineered DSAEK grafts using cultured HCECs.
  • To explore the potential of HCEC precursors for corneal regeneration.

Main Methods:

  • Cultured HCECs were seeded onto various substrates (amniotic membrane, corneal stroma, collagen sheets) to create HCEC sheets for DSAEK grafts.

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Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
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Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

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Last Updated: May 14, 2026

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
05:20

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

  • Sphere-forming assay was utilized for isolating and expanding HCEC precursors.
  • HCEC sheets were transplanted onto rabbit corneas following DSAEK, with comparisons to control groups.

    • Main Results:

    • Engineered HCEC sheets demonstrated 75%-95% of the pump function of donor corneas.
    • Transplanted HCEC sheets resulted in clear corneas in rabbits, unlike the severe edema in controls.
    • The sphere-forming assay enabled mass production of human corneal endothelial precursors.

    Conclusions:

    • Cultured HCEC sheets transplanted via DSAEK can effectively dehydrate corneas in vivo, indicating feasibility for treating endothelial dysfunction.
    • Corneal endothelial precursors generated via sphere-forming assay represent a promising strategy for corneal endothelial regeneration.
    • Tissue engineering of HCECs offers a viable alternative to donor corneas for DSAEK procedures.