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Christopher J Horton1, Midori Mitui, Nora K Leos

  • 1Children's Medical Center, 1935 Medical District Dr., Dallas, TX 75235, United States. christopher.horton@childrens.com

Molecular and Cellular Probes
|January 29, 2013
PubMed

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...