Related Experiment Video
Updated: May 14, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
C/D box sRNA, CRISPR RNA and tRNA processing in an archaeon with a minimal fragmented genome
Hagen Richter1, Sabine Mohr, Lennart Randau
1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, 35037 Marburg, Germany.
Abstract:
The analysis of deep sequencing data allows for a genome-wide overview of all the small RNA molecules (the 'sRNome') that are present in a single organism. In the present paper, we review the processing of CRISPR (clustered regularly interspaced short palindromic repeats) RNA, C/D box sRNA (small non-coding RNA) and tRNA in Nanoarchaeum equitans. The minimal and fragmented genome of this tiny archaeon permits a sequencing depth that enables the identification of processing intermediates in the study of RNA processing pathways. These intermediates include circular C/D box sRNA molecules and tRNA half precursors.
More Related Videos
Related Concept Videos
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Diversity of Archaea III
The Antiviral System of Bacteria and Archaea: CRISPR
Viruses of Archaea
Nucleoid
CRISPR

