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Related Experiment Video

Updated: May 14, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Tissue microarrays: construction and use.

Helen Remotti1

  • 1Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA. her2007@columbia.edu

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2013
PubMed
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Tissue microarrays (TMAs) provide a cost-effective method for high-throughput analysis of numerous tissue samples. This chapter details TMA construction for studying pancreatic neoplasms using formalin-fixed paraffin-embedded tissue.

Area of Science:

  • Pathology and Laboratory Medicine
  • Oncology Research
  • Biotechnology

Background:

  • Tissue microarrays (TMAs) are essential for high-throughput analysis of biological samples.
  • Analyzing multiple tissue samples efficiently is crucial for understanding diseases like pancreatic cancer.
  • Formalin-fixed paraffin-embedded (FFPE) tissues are a common resource in pathology.

Purpose of the Study:

  • To outline the methodology for constructing tissue microarrays (TMAs).
  • To provide guidance for analyzing pancreatic neoplasms using TMAs.
  • To demonstrate the utility of TMAs for formalin-fixed paraffin-embedded (FFPE) tissue analysis.

Main Methods:

  • Tissue cores are selected from donor paraffin-embedded tissue blocks.
  • Cores are arrayed into a recipient TMA block for positional encoding.

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SpOT the Correct Tissue Every Time in Multi-tissue Blocks
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SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

Related Experiment Videos

Last Updated: May 14, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

SpOT the Correct Tissue Every Time in Multi-tissue Blocks
06:53

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

  • The described technique is specifically for formalin-fixed paraffin-embedded (FFPE) tissues.

    • Main Results:

    • TMAs facilitate parallel analysis of numerous tissue samples.
    • Various analytical techniques can be applied to constructed TMAs, including staining and hybridization.
    • The method is cost-effective for large-scale tissue analysis.

    Conclusions:

    • TMA construction is a valuable technique for high-throughput tissue analysis.
    • This method is particularly useful for the study of pancreatic adenocarcinomas and pre-invasive lesions.
    • The chapter provides a practical guide for TMA creation using FFPE tissues.