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Related Concept Videos

Regulation of Pulse01:20

Regulation of Pulse

Pulse regulation involves physiological mechanisms that ensure adequate blood flow throughout the body. The heartbeat, regulated by the autonomic nervous system, is influenced by hormonal balance, physical activity, and emotional state.
Pulse rhythm01:30

Pulse rhythm

Pulse rhythm refers to the pattern of pulsations within specific intervals, offering valuable insights into the regularity or irregularity of the heart's beats as observed through the pattern of pulsation within specific intervals. A regular pulse exhibits a consistent heart rate with uniform waveforms and pulsation force, variations of which can be classified as normal, weak, or bounding.
Conversely, an irregular pulse pattern is termed dysrhythmia, stemming from disruptions in cardiac muscle...
Pulse01:05

Pulse

The pulse is one of the most fundamental physiological indicators of the body's cardiovascular health. It is the rhythmic expansion and contraction of the arterial walls in response to the pressure generated by the heart's pumping action.
Pulse Rate and its Significance
Pulse rate, often measured in beats per minute (bpm), reflects the heart rate (HR), which is influenced by numerous factors such as stress, physical activity, and hormonal changes. A normal resting adult pulse rate falls between...
Pulse01:16

Pulse

When the heart pumps blood out, arterial elastic fibers play a crucial role in sustaining a high-pressure gradient. They expand to accommodate the received blood and then recoil - a process known as the pulse that can be either manually palpated or electronically quantified. Despite a reduction in its effect with increased distance from the heart, elements of the pulse's systolic and diastolic components persist, observable even at the arteriole level.
The pulse serves as a clinical indicator...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Pulse amplitude and quality01:17

Pulse amplitude and quality

Pulse amplitude is a crucial indicator of cardiac health because it provides valuable insights into the strength of left ventricular contractions and the overall uniformity of blood circulation within the vasculature. The strength of the pulse is directly related to the force with which the heart contracts and the volume of blood being pumped.
A weak or absent pulse may indicate reduced cardiac output or poor left ventricular contraction, which can be signs of cardiovascular dysfunction or...

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Developing a Behavioral Box for Assessing Prepulse Inhibition and Neural Activity in Psychiatric Animal Models
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Published on: July 22, 2025

Bomb Pulse Biology.

Miranda J Sarachine Falso1, Bruce A Buchholz

  • 1Center for Accelerator Mass Spectrometry, Mail stop L-397, Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, CA 94551 USA.

Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
|January 30, 2013
PubMed
Summary
This summary is machine-generated.

The bomb pulse carbon-14 (¹⁴C) method revolutionizes cell biology by enabling precise cell turnover studies. This technique focuses on specific cell types and biomolecules, overcoming limitations of older methods for accurate cellular analysis.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Radiochemistry

Background:

  • The (14)C bomb-pulse technique has become crucial for fundamental cell biology research over the past decade.
  • Traditional bulk tissue measurements are less relevant due to rapid turnover of major carbon components (proteins, lipids, carbohydrates).
  • Current research prioritizes investigating cell turnover in specific, specialized cell types.

Purpose of the Study:

  • To detail methodologies for utilizing the (14)C bomb-pulse in modern cell biology.
  • To address the challenges of isolating specific cell types and biomolecules for analysis.
  • To ensure high purity and accuracy in measurements using accelerator mass spectrometry (AMS).

Main Methods:

  • Utilizing fresh or frozen tissue samples, avoiding contamination from fixed or preserved specimens.
  • Employing cell or nuclear surface markers for cell sorting, often via fluorescence-activated cell sorting (FACS).
  • Isolating specific biomolecules (proteins, DNA) to high purity (<40 micrograms of carbon) and confirming purity using methods like UV absorbance.

Main Results:

  • Established protocols for separating specific proteins and DNA from complex biological samples.
  • Developed strategies to prevent contamination during sample preparation and isolation.
  • Demonstrated the feasibility of using AMS for analyzing minute quantities of carbon in purified biomolecules.

Conclusions:

  • The (14)C bomb-pulse method, coupled with advanced separation and purification techniques, is a powerful tool for cell turnover studies.
  • Accurate analysis of specific cell types and biomolecules is now achievable with high precision.
  • Methodological advancements enable the study of cellular processes at a molecular level with unprecedented detail.