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In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

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Published on: January 29, 2018

SUMO modification of menin.

Zi-Jie Feng1, Buddha Gurung, Guang-Hui Jin

  • 1Department of Basic Medical Sciences, Medical College, Xiamen University Xiamen, Fujian, China 361005 ; Abramson Family Cancer Research Institute, Department of Cancer Biology, Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine 421 Curie Blvd., Philadelphia, PA 19104, USA.

American Journal of Cancer Research
|January 30, 2013
PubMed
Summary

This study reveals that menin undergoes SUMOylation, a post-translational modification. This modification, involving lysine 591, impacts menin

Keywords:
K591RMeninSUMO1SUMOylation

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Biology

Background:

  • Menin functions as a context-dependent tumor suppressor and promoter.
  • Menin's role in epigenetic regulation of gene transcription is established.
  • While menin phosphorylation is known, other post-translational modifications remain largely uncharacterized.

Purpose of the Study:

  • To investigate potential post-translational modifications of menin beyond phosphorylation.
  • To determine if menin undergoes SUMOylation and identify the specific sites involved.
  • To assess the functional consequences of menin SUMOylation on its tumor-suppressive/promotive activities.

Main Methods:

  • In vivo and in vitro SUMOylation assays were performed.
  • Site-directed mutagenesis was used to identify SUMOylation sites (K591R mutant).
  • SUMOylation levels were assessed using Western blotting and SUMO proteases.

Main Results:

  • Menin was found to be SUMOylated by SUMO1 both in vivo and in vitro.
  • Lysine 591 (K591) was identified as a key SUMOylation site.
  • Mutation of K591 partially inhibited SUMOylation, suggesting additional sites exist.
  • SUMOylation of menin did not abolish its ability to regulate cell proliferation or target gene expression.

Conclusions:

  • Menin undergoes SUMOylation, a novel post-translational modification.
  • SUMOylation at K591 is a significant modification, but not the sole site.
  • Despite SUMOylation, menin retains its regulatory functions in cell proliferation and gene expression, indicating functional resilience.