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Updated: May 14, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
Seung Woo Cho1, Sojung Kim, Jong Min Kim
1National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea.
Nature Biotechnology
|January 31, 2013
Abstract:
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
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