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Updated: May 14, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Published on: September 8, 2010
DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells
E I Cortés-Gutiérrez1, M I Dávila-Rodríguez, J L Fernández
1División de Genética, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS, Monterrey, México. elvacortes@cibinmty.net
Abstract:
DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

