Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cilia to basement membrane signaling is a biomechanical driver in models of autosomal dominant polycystic kidney disease.

The Journal of clinical investigation·2026

Same author

Evaluation of the contribution of trio-exome sequencing in selected prenatal indications.

Frontiers in genetics·2026

Same author

HNF1B integrates signals in a feed-forward loop driving kidney disease progression.

Science (New York, N.Y.)·2026

Same author

Genetic screening in kidney transplant candidates.

Clinical kidney journal·2026

Same author

Unequal mitochondrial segregation promotes asymmetric fates during neurogenesis.

Nature communications·2025

Same author

A low-level Cdkn1c/p57<sup>kip2</sup> expression in spinal progenitors drives the transition from proliferative to neurogenic modes of division.

EMBO reports·2025

Search research articles

Related Experiment Video

Updated: May 14, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Hepatocyte nuclear factor 1β controls nephron tubular development.

Filippo Massa¹, Serge Garbay, Raymonde Bouvier

¹Expression Génique, Développement et Maladies (EGDM) Team, INSERM U1016,CNRS UMR 8104, Université Paris-Descartes. Institut Cochin; Département deGénétique et Développement, 75014 Paris, France.

Development (Cambridge, England)

|January 31, 2013

Summary

Hepatocyte nuclear factor 1β (HNF1β) is crucial for kidney development. Its inactivation in mice causes severe tubular defects, revealing its role in forming S-shaped bodies and regulating key developmental genes.

More Related Videos

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Published on: December 21, 2016

Related Experiment Videos

Last Updated: May 14, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Published on: December 21, 2016

Area of Science:

Developmental Biology
Molecular Biology
Genetics

Background:

Nephron morphogenesis is vital for kidney function, involving complex formation of glomeruli and tubules.
Hepatocyte nuclear factor 1β (HNF1β) is a transcription factor critical for kidney development.
Mutations in HNF1B are linked to renal developmental pathologies, but underlying mechanisms remain unclear.

Purpose of the Study:

To elucidate the role of HNF1β in murine nephrogenesis.
To investigate the impact of HNF1β inactivation on early kidney development and gene expression.

Main Methods:

Inactivation of Hnf1b in murine metanephric mesenchyme.
Analysis of nephron structure and S-shaped body morphology.
Gene expression analysis of key developmental regulators.

Main Results:

Hnf1b inactivation led to the absence of proximal, distal, and Henle's loop segments, resulting in glomeruli connected directly to collecting ducts via short tubules.
Deformed S-shaped bodies lacking a critical bulge were observed, preventing proximal tubule and Henle's loop formation.
Downregulation of Irx1, Osr2, Pou3f3, and defective Notch activation via Dll1 were noted in HNF1β-deficient precursors.

Conclusions:

HNF1β plays a hierarchical role in regulating genes essential for renal tubule development.
This study identifies a novel structural and functional role for S-shaped bodies in initiating tubule formation.
Defective HNF1β function disrupts normal nephron development, contributing to congenital renal anomalies.