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Updated: May 14, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Surveillance and evidence of contamination in hospital environment from meticillin and vancomycin-resistant microbial
R Squeri1, O C Grillo, V La Fauci
1Department of Hygiene, Preventive Medicine and Public Health, University Hospital G. Martino, Messina, Italy. raffaele.squeri@unime.it
Introduction:
Direct contact is undoubtedly the main means of transmission of hospital infections. An investigative study was therefore conducted to assess workplace surfaces at risk from microbial contamination.
Methods:
The study was conducted using swabs and contact slides placed on the palms of healthcare workers during their routine patient care and on workplace surfaces (e.g. telephones, computers, medication trolleys, taps) in treatment rooms, operating theatres and wards. Disposable swabs were used for rapid screening and read with a bioluminometer. At the same time, a sample was taken from those testing positive using a contact slide. The samples testing positive for Staphylococci underwent identification to assess resistance to meticillin-resistant Staphylococcus aureus (MRS/MRSA) and to vancomycin (VISA/VRSA).
Results:
Meticillin-resistant Staphylococcus strains were found on 14.7% (20/136) of samples taken from the hands of workers and 35.7% (15/42) of those from hospital surfaces. An even higher resistance to meticillin and/or vancomycin than that found for S. aureus was identified in nosocomial strains of coagulase negative staphylococci, including S. epidermidis and S. haemolyticus.
Conclusion:
The study concludes that there is thus a need for greater care in complying with procedures designed and support for surveillance to reduce the risk of infection.
Insights
Hospital surfaces and healthcare workers' hands pose a significant risk for transmitting infections. Meticillin-resistant Staphylococcus strains were frequently detected on both, highlighting the urgent need for improved hygiene protocols to reduce hospital-acquired infections.
Area of Science:
- Infection Control
- Microbiology
- Healthcare Epidemiology
Background:
- Hospital-acquired infections are primarily transmitted through direct contact.
- Assessing microbial contamination on healthcare workplace surfaces is crucial for infection control.
Purpose of the Study:
- To evaluate the risk of microbial contamination on healthcare workers' hands and workplace surfaces.
- To identify the prevalence of resistant Staphylococcus strains in a hospital environment.
Main Methods:
- Swabs and contact slides collected from healthcare workers' hands and surfaces (telephones, computers, trolleys, taps).
- Rapid screening using bioluminometry, followed by identification of positive Staphylococci samples.
- Assessment of resistance to meticillin-resistant Staphylococcus aureus (MRSA) and vancomycin (VRSA).
Main Results:
- Meticillin-resistant Staphylococcus strains found on 14.7% of hand samples and 35.7% of surface samples.
- Higher resistance rates to meticillin and/or vancomycin observed in nosocomial coagulase-negative staphylococci compared to S. aureus.
- Specific resistant strains identified include S. epidermidis and S. haemolyticus.
Conclusions:
- A significant presence of resistant Staphylococcus strains on hands and surfaces necessitates enhanced infection control measures.
- Increased adherence to hygiene procedures and robust surveillance are vital to mitigate infection risks.
- Focus on preventing transmission of multidrug-resistant organisms in healthcare settings is paramount.
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