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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
A mutation 'hot spot' in the Schmallenberg virus M segment.
Melina Fischer1, Bernd Hoffmann1, Katja V Goller1
1Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
The Journal of General Virology
|February 1, 2013
Summary
Schmallenberg virus (SBV) genome variability was studied in German livestock. Researchers identified a mutation
Area of Science:
- Virology
- Genomics
- Veterinary Medicine
Background:
- Schmallenberg virus (SBV), an orthobunyavirus, emerged in Europe in 2011.
- Previous studies focused on SBV's phylogeny, clinical signs, and epidemiology.
- Detailed sequence data for SBV's S and M segments, crucial for virus-host interactions, were lacking.
Purpose of the Study:
- To investigate the S and M genome segments of Schmallenberg virus (SBV).
- To analyze sequence variability in SBV field isolates from different German regions and host species.
Main Methods:
- Sequence analysis of S and M segments from 24 SBV-positive field samples (sheep, cattle, goat).
- Phylogenetic analysis to compare SBV genome segments with other Simbu serogroup viruses.
Main Results:
- Overall SBV genome variability was not dependent on geographic region or host species.
- A mutation 'hot spot' within the glycoprotein Gc (encoded by the M segment) was identified for the first time.
- Sequence data for S and M segments were characterized, providing insights into virus-host interactions.
Conclusions:
- SBV exhibits consistent genome variability across different regions and hosts in Germany.
- The identified mutation 'hot spot' in the Gc protein is a key finding for understanding SBV evolution and pathogenesis.
- This study provides essential genomic data for SBV, aiding future research on virus-host interactions and control strategies.
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