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Related Experiment Video

Updated: May 14, 2026

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
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A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

Single cell membrane poration by bubble-induced microjets in a microfluidic chip.

Z G Li1, A Q Liu, E Klaseboer

  • 1Division of Microelectronics, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, 639798, Singapore.

Lab on a Chip
|February 1, 2013
PubMed
Summary

Researchers created a microjet from a laser-induced bubble to rupture single cell membranes. This method precisely porates cells, with pore size depending on bubble dynamics and proximity.

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Area of Science:

  • Biophysics
  • Microfluidics
  • Cell Biology

Background:

  • Precise manipulation of individual cells is crucial for various biological studies.
  • Laser-induced bubble dynamics offer potential for non-invasive cellular manipulation.
  • Existing methods for cell membrane poration can lack precision or scalability.

Purpose of the Study:

  • To demonstrate membrane poration of single suspension cells using a laser-induced microjet.
  • To investigate the relationship between cavitation bubble dynamics and cell membrane rupture.
  • To establish a method for controlled and localized cell membrane poration.

Main Methods:

  • A laser-induced bubble was generated near a single, trapped suspension cell within a microfluidic chip.
  • The asymmetrical collapse of the bubble created a high-speed liquid microjet impacting the cell.
  • Membrane poration was assessed by trypan blue uptake, with time-resolved diffusion studies.

Main Results:

  • Successful membrane poration of myeloma cells was achieved via microjet impact.
  • Trypan blue diffusion showed a strong dependence on the bubble's stand-off distance from the cell.
  • Shorter distances resulted in increased dye penetration, indicating larger membrane pores.

Conclusions:

  • Laser-induced bubble collapse provides a mechanism for controlled single-cell membrane poration.
  • The method is fast, repeatable, and allows for localized membrane rupture.
  • This technique has potential applications in cell analysis and manipulation.