Lower gut nitrogen supply does not affect apparent ileal digestibility of nitrogen or amino acids in growing pigs

An implicit assumption in measures of ileal digestibility (ID) to estimate bioavailability of AA and N is that ID is not influenced by lower gut N metabolism. The absorption of nitrogenous compounds from the lower gut, derived from fermentative AA catabolism, may have an impact on N metabolism and microbial AA synthesis in the upper gut as a result of CO(NH(2))(2) recycling. The objective of this trial was to determine the apparent ID of N and AA in growing pigs fed a corn (Zea mays) starch and soybean (Glycine max) meal-based diet and receiving an infusion of N into the caecum at 40% of N intake. Eight pigs (initial BW of 23.3 ± 0.55 kg) were fitted with simple T-cannulas in the ileum and cecum and randomly assigned to 1 of 3 continuous cecal infusion treatments [saline, sodium caseinate, or CO(NH(2))(2)] according to a crossover design with 3 periods. Digesta samples were collected and pooled per pig for each 2-d period, freeze-dried, ground, and analyzed for DM, OM, total N, and AA. Lower gut N supply did not affect apparent ID of DM, OM, total N (85.4, 83.4, and 82.7 ± 1.74%; P = 0.31), or any AA (e.g., 90.1, 89.0, and 89.9 ± 1.08% for Lys; P = 0.70) for saline, casein, and CO(NH(2))(2) treatments, respectively. Apparent ID may be an insufficiently sensitive measure to determine effects of lower gut N metabolism on N absorption from the lower gut and subsequent recycling into the upper gut.