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Updated: May 14, 2026

Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
Published on: February 22, 2012
The human papillomavirus type 8 E6 protein interferes with NOTCH activation during keratinocyte differentiation
Jordan M Meyers1, Jennifer M Spangle, Karl Munger
1Division of Infectious Disease, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Abstract:
Cutaneous β-human papillomavirus (β-HPV) E6 proteins inhibit NOTCH signaling by associating with the transcriptional coactivator MAML1. NOTCH has tumor suppressor activities in epithelial cells and is activated during keratinocyte differentiation. Here we report that HPV type 8 (HPV8) E6 subverts NOTCH activation during keratinocyte differentiation by inhibiting RBPJ/MAML1 transcriptional activator complexes at NOTCH target DNA. NOTCH inhibition impairs epithelial differentiation and may thus contribute to β-HPV replication and viral oncogenesis.
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