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Related Experiment Video

Updated: May 14, 2026

Optogenetic Activation of Intrinsic Cardiac Autonomic Neurons in Excised Perfused Mouse Hearts
08:29

Optogenetic Activation of Intrinsic Cardiac Autonomic Neurons in Excised Perfused Mouse Hearts

Published on: March 28, 2025

Cardiac optogenetics.

Oscar J Abilez1

  • 1Bio-X Program, Stanford University, Stanford, CA 94305, USA. ojabilez@stanford.edu

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|February 1, 2013
PubMed
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Optogenetically modified human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) can be controlled with light. This light-based activation and inhibition of hiPSC-CM offers potential for precise cardiac rhythm synchronization in future therapies.

Area of Science:

  • Cardiology
  • Stem Cell Biology
  • Optogenetics

Background:

  • Therapies using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) require methods to prevent arrhythmias.
  • Optogenetic tools offer potential for precise control of cellular activity.

Purpose of the Study:

  • To investigate the feasibility of optogenetically controlling hiPSC-CM using light-activated proteins.
  • To assess the functional response of modified hiPSC-CM to optical stimulation.

Main Methods:

  • Human induced pluripotent stem cells (hiPSCs) were transduced with lentiviral vectors encoding channelrhodopsin-2 (ChR2) and halorhodopsin (NpHR1.0).
  • Directed differentiation was used to produce wildtype (hiPSC(WT)-CM) and optogenetically modified (hiPSC(ChR2/NpHR)-CM) cardiomyocytes.
  • Both cell types were subjected to electrical and optical stimulation in vitro.

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Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts
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Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts

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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
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Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Published on: March 5, 2020

Related Experiment Videos

Last Updated: May 14, 2026

Optogenetic Activation of Intrinsic Cardiac Autonomic Neurons in Excised Perfused Mouse Hearts
08:29

Optogenetic Activation of Intrinsic Cardiac Autonomic Neurons in Excised Perfused Mouse Hearts

Published on: March 28, 2025

Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts
08:43

Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts

Published on: August 26, 2021

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
12:52

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Published on: March 5, 2020

Main Results:

  • hiPSC(WT)-CM and hiPSC(ChR2/NpHR)-CM responded similarly to electrical stimulation, exhibiting comparable contractility.
  • Only hiPSC(ChR2/NpHR)-CM demonstrated synchronized activation and inhibition in response to optical stimulation (480 nm for ChR2, 580 nm for NpHR1.0).
  • Light-sensitive proteins enabled in vitro optical control of hiPSC-CM function.

Conclusions:

  • Optogenetic modification of hiPSC-CM with ChR2 and NpHR1.0 allows for precise optical control.
  • In vivo optical stimulation could enable targeted synchronization of implanted hiPSC-CM with cardiac rhythms.
  • This approach holds promise for advanced cardiac regenerative therapies, mitigating arrhythmia risks.