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Updated: May 14, 2026

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
Published on: July 3, 2015
Single molecule tracking of P-glycoprotein in live cells reveals dynamic heterogeneity
Philip P Cheney1, Michael D Stachler, Michelle K Knowles
1Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA. Philip.Cheney@du.edu
Abstract:
P-glycoprotein transports chemotherapy drugs from the plasma membrane and allows cancer cells to survive treatment. We transiently transfected PGP labeled with enhanced green fluorescent protein (PGP-EGFP) into MES-SA cells and used single molecule tracking techniques to characterize the dynamics on the surface of live cells. PGP exhibits freely diffusive behavior at short times and is confined at long times with a transition to anomalous diffusion at 0.7 s.
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