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Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:

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Related Experiment Video

Updated: May 14, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Visualization of high resolution spatial mass spectrometric data during acquisition.

Mathew Thomas1, Brandi S Heath, Julia Laskin

  • 1Pacific Northwest National Laboratory, Richland, WA 99354, USA. mathew.thomas@pnnl.gov

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|February 1, 2013
PubMed
Summary

New software, MSI QuickView, simplifies analyzing complex spatial mass spectrometry data. This tool enhances visualization and interpretation of molecular distributions in tissues and cells, improving research efficiency.

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging
09:43

Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging

Published on: January 10, 2025

Area of Science:

  • Biomedical imaging
  • Analytical chemistry
  • Data science

Background:

  • Mass Spectrometric Imaging (MSI) generates high-resolution molecular maps from tissue and cell data.
  • MSI data is large and complex, requiring advanced analytical tools for interpretation.
  • Current methods for MSI data analysis are fragmented and inefficient.

Purpose of the Study:

  • To develop and apply a novel software toolkit for processing, visualizing, and analyzing spatial mass spectrometry data.
  • To create an integrated solution that overcomes limitations of existing MSI data analysis workflows.
  • To enhance the efficiency and accessibility of MSI data interpretation.

Main Methods:

  • Development of an interactive software toolkit named MSI QuickView.
  • Implementation of capabilities for processing, visualizing, and querying spatial mass spectrometry data.
  • Generation of 2D ion density maps and heat maps from selected spectral data.

Main Results:

  • MSI QuickView enables direct processing and visualization of MSI data.
  • The software facilitates the generation of 2D molecular maps and heat maps.
  • It allows for the identification of specific spectral peaks within the complex datasets.
  • The toolkit streamlines the analysis workflow compared to previous multi-software approaches.

Conclusions:

  • MSI QuickView represents a significant advancement in the analysis of spatial mass spectrometry data.
  • The software provides an efficient and integrated platform for MSI data interpretation.
  • This tool will accelerate discoveries in molecular imaging of biological samples.