Computerized three-dimensional reconstructions of serial sections in electron microscopy
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1Institute of Anatomy and Cytobiology, Justus-Liebig-University, Giessen, Fed. Rep. of Germany.
Ultramicroscopy
|January 1, 1990
Abstract:
To improve reconstructive 3D electron microscopy novel methods are discussed to represent and process serial section images in a cuberille environment. This includes the analysis of the transfer characteristics of the image detection system, the use of laser-induced fiducials for deformation correction and alignment, the control of section thickness by EELS and the use of ESI to image thick sections.