Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal-Organic Frameworks.

Advanced functional materials·2026
Same author

ASO Visual Abstract: KRAS Mutation Allele Frequency Dynamics in Plasma Extracellular Vesicles: Associations with Survival in Localized Pancreatic Adenocarcinoma.

Annals of surgical oncology·2025
Same author

ASO Author Reflections: Beyond CA19-9-Integrating Extracellular Vesicle (EV)-KRAS Dynamics into Neoadjuvant Therapy Assessment for Pancreatic Cancer.

Annals of surgical oncology·2025
Same author

KRAS Mutation Allele Frequency Dynamics in Plasma Extracellular Vesicles: Association with Survival in Localized Pancreatic Adenocarcinoma.

Annals of surgical oncology·2025
Same author

From mechanisms to precision medicine: the role of organoids in studying the gut microbiota-tumor microenvironment axis.

Frontiers in microbiology·2025
Same author

Sequencing-free, joint single-EV profiling of DNA and protein cargos enables accurate cancer detection at early stages.

Lab on a chip·2025

Related Experiment Video

Updated: May 14, 2026

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

Viable circulating tumor cell enrichment by flexible micro spring array.

Ramdane A Harouaka1, Ming-Da Zhou, Tim Y Yeh

  • 1Department of Bioengineering and Material Research Institute, The Pennsylvania State University, University Park, PA 16802, USA.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|February 1, 2013
PubMed
Summary
This summary is machine-generated.

This study presents a high-throughput platform for isolating circulating tumor cells (CTCs) from blood. The versatile device efficiently enriches viable CTCs using size-based micro-separation, crucial for cancer research.

More Related Videos

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
10:12

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Published on: May 15, 2018

Related Experiment Videos

Last Updated: May 14, 2026

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
10:12

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Published on: May 15, 2018

Area of Science:

  • Biomedical Engineering
  • Oncology
  • Cell Biology

Background:

  • Circulating tumor cells (CTCs) are critical biomarkers for cancer detection, prognosis, and treatment monitoring.
  • Isolating viable CTCs from whole blood presents significant technical challenges due to their low abundance and fragility.

Purpose of the Study:

  • To develop and validate a high-throughput, versatile platform for the efficient isolation of viable circulating tumor cells (CTCs) from large blood volumes.
  • To leverage size-based micro-separation for CTC enrichment, preserving cellular integrity and proliferative capacity.

Main Methods:

  • A micro-scale, size-based separation system was engineered for high-throughput blood processing.
  • The platform utilizes the size difference between CTCs and normal blood cells for enrichment.
  • Testing involved model systems using established cancer cell lines spiked into whole blood.

Main Results:

  • The platform achieved a 90% capture efficiency for CTCs from 7.5 mL of whole blood.
  • Enrichment factors exceeding 10^4 were demonstrated.
  • Over 80% cell viability was maintained post-isolation, with processing times of approximately ten minutes.
  • The system showed no incidence of clogging during testing.

Conclusions:

  • The developed platform offers a rapid, efficient, and versatile solution for isolating viable CTCs.
  • This technology has significant potential for advancing liquid biopsy applications in oncology.
  • The high capture efficiency and preserved viability of CTCs are crucial for downstream molecular and functional analyses.