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Updated: May 14, 2026
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
[The ALV-A/B specific antibodies correlation between ELISA and IFA detection in chicken serum]
Xue Li1, De-Qing Li, Peng Zhao
1College of Animal Science and Technology, Shandong Agricultural University, Shandong Engineering Research Center for Animal Disease Control and Prevention, Taian 271018, China. lixue880209@163.com
Abstract:
To study the correlation between ELISA and IFA tests in detection of ALV-A/B antibody in chicken sera, ELSA S/P values and IFA titers for different serum samples were measured and statistically analyzed. The results indicated that there was a strong positive correlation between ELISA S/P values and IFA titers (r = 0.97435, P < 0.001). Because the positive correlation between ELISA and IFA was so strong and antibody positive rates were identical in two tests, it suggested that IFA could be used as a alternative method to replace ELISA kit when only limited numbers of samples to be tested to reduce the cost and increase the sensitivity.
