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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Published on: September 20, 2016
Selective fluorescence detection of small-molecule-binding proteins by using a dual photoaffinity labeling system
Kaori Sakurai1, Rika Yamada, Ayumi Okada
1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588, Japan. sakuraik@cc.tuat.ac.jp
Abstract:
PHOTO OPPORTUNITY: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.
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