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Related Concept Videos

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
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Renewing the assault on mRNA.

Jack McCain

    Biotechnology Healthcare
    |February 2, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Mammalian cells use RNA interference to defend against intruders by degrading double-stranded RNA. This natural defense mechanism, utilizing small interfering RNA, shows promise for novel disease treatments.

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    Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
    09:20

    Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

    Published on: April 11, 2022

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Mammalian cells possess defense mechanisms against foreign genetic material.
    • Double-stranded RNA is recognized as a threat by cellular systems.
    • RNA interference (RNAi) is a recently discovered pathway for gene silencing.

    Purpose of the Study:

    • To explore the potential of RNA interference as a therapeutic strategy.
    • To compare RNA interference with previously studied gene-silencing methods like antisense and ribozymes.
    • To assess if RNA interference represents a significant advancement or a variation of existing themes.

    Main Methods:

    • Investigated the cellular response to double-stranded RNA.
    • Characterized the mechanism of RNA interference, including the role of small interfering RNA.
    • Compared the gene-silencing targets of RNA interference, antisense, and ribozymes (messenger RNA).

    Main Results:

    • Mammalian cells degrade double-stranded RNA via RNA interference.
    • Small interfering RNA molecules are key components in identifying and destroying foreign RNA.
    • RNA interference targets messenger RNA for gene silencing, similar to antisense and ribozyme therapies.

    Conclusions:

    • RNA interference is a potent natural defense mechanism with therapeutic potential.
    • The therapeutic applicability of RNA interference warrants further investigation.
    • RNA interference shares similarities with older gene-silencing techniques, raising questions about its novelty.