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Related Experiment Video

Updated: May 14, 2026

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

Atad3 function is essential for early post-implantation development in the mouse.

Tobias Goller1, Ursula K Seibold, Elisabeth Kremmer

  • 1LIMES Institute, Program Unit Molecular Cell and Immune Biology, University of Bonn, Bonn, Germany.

Plos One
|February 2, 2013
PubMed
Summary

Mice lacking the mitochondrial AAA+-ATPase ATAD3 show embryonic lethality around E7.5 due to impaired trophoblast development and mitochondrial biogenesis, highlighting ATAD3

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Area of Science:

  • Mitochondrial biology
  • Developmental biology
  • Mammalian embryogenesis

Background:

  • The mitochondrial AAA+-ATPase ATAD3 is known to regulate mitochondrial and ER dynamics and is essential for development in C. elegans.
  • Its role in mammalian development, particularly during early embryogenesis, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of ATAD3 in mammalian development by analyzing a mouse model lacking the Atad3 gene.
  • To determine the specific developmental stage and cellular processes affected by Atad3 deficiency.

Main Methods:

  • Generation and analysis of an Atad3 deficient mouse line.
  • Phenotypic characterization of Atad3 deficient embryos, focusing on developmental milestones and trophoblast lineage.
  • Biochemical analysis of ATAD3 protein complexes within mitochondria.

Main Results:

  • Atad3 deficient embryos exhibit embryonic lethality around embryonic day E7.5.
  • The lethality is associated with severe growth retardation and defective trophoblast development post-implantation.
  • Biochemical analysis revealed ATAD3 exists in five distinct native mitochondrial protein complexes, suggesting multifaceted roles.

Conclusions:

  • Murine Atad3 is essential for early post-implantation development, specifically for mitochondrial biogenesis in trophoblast stem cells and differentiating trophoblasts.
  • ATAD3 plays a critical role in supporting the high energy demands of early embryonic development through oxidative phosphorylation.
  • The diverse protein complexes involving ATAD3 indicate its complex involvement in mitochondrial architecture and function.