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[HPLC fingerprint of Liuwei Dihuang condensed pills]
Xin-Biao Gao1, Lei Sun, Shan-Yi Qiao
1Key Laboratory of Structure Based Drug Design & Discovery of Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. gaoxinzhu653808@163.com
Objective:
To establish HPLC fingerprints of Liuwei Dihuang condensed pills.
Method:
Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile (containing 0.05% phosphoric) -water (containing 0.05% phosphoric) as the mobile phase. The column temperature was set at 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was 276 nm (0-10 min), 236 nm (10-40 min) and 276 nm (40-60 min). The sample size was 20 microL. Chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.
Result:
Good precision, stability and repeatability were proved. Q-TOF-MS-IDA-MS/ MS method was adopted for qualitative determination of eighteen chromatographic peaks. Ten batches of Liuwei Dihuang condensed pills were determined with the method, and their similarities were above 0. 96.
Conclusion:
The study lays a foundation for the overall quality evaluation of Liuwei Dihuang condensed pills.