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Updated: May 14, 2026
Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus
Published on: March 28, 2025
[Cloning, expression and immunodiagnostic evaluation of enolase from Echinococcus granulosus]
Ying Wang1, Jing Zhang, Zhong-Ying Yuan
1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, Ministry of Health, China.
Objective:
To clone and express EgEno gene of Echinococcus granulosus, and to investigate the immunogenicity and diagnostic value of recombinant EgEno.
Methods:
Total RNAS of E. granulosus was extracted and reversedly transcripted to cDNA. EgEno gene was amplified from cDNA and inserted into vector pET28a. The recombinant plasmid pET28a-EgEno was transformed into E. coli BL21 (DE3) for expression under the induction of IPTG. The expressed product was identified by SDS-PAGE and Western blotting. The purified recombinant EgEno protein was detected by ELISA with the sera of cystic echinococcosis patients, healthy persons and other patients.
Results:
The EgEno gene was successfully amplified from cDNA of E. granulosus and a fusion protein was expressed in E. coli BL21 (DE3). The molecular weight of the expressed protein was around 50 kDa. The result of Western blotting indicated that the antigenicity of the protein was specific. The sensitivity of diagnosis by ELISA for cystic echinococcosis was 81.25%.
Conclusion:
EgEno of E. granulosus is cloned and expressed in E. coli BL21 (DE3) successfully, which might be used as a candidate antigen of immunodiagnosis for cystic echinococcosis.
