Pathogen diagnosis of children sepsis by LAMP technology
1Shenzhen Longhua People's Hospital, ShenZhen 518000, China. zyclh121@126.com
Objective:
To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis.
Methods:
The primers were designed and synthesized based on 16S rRNA gene of Staphylococcus aureus. Four specimens of Staphylococcus aureus, 16 specimens of coagulase-negative Staphylococci, 2 specimens of Enterococci, 3 specimens of Streptococcus, 1 specimen of Micrococcus, 3 specimens of Escherichia coli, 4 specimens of Klebsiella pneumoniae, 3 specimens of Pseudomonas aeruginosa, 2 specimens of Enterobacter cloacae, and 5 specimens of Acinetobacter were tested by loop-mediated isothermal amplification (LAMP) assay. A total of 118 clinical specimens of sepsis and non-sepsis were collected and detected with both LAMP assay and blood culture.
Results:
By designing primers specific for Staphylococcus aureus, specimens containing different kinds of pathogens were carried out by LAMP assay, and our data showed LAMP technology for the specific detection of Staphylococcus aureus in samples was successfully established. All clinical specimens of sepsis and non-sepsis were tested by both blood cultures and LAMP, and our data showed that compared wit blood culture method, the LAMP technology showed significantly high detection rate (P <0.01).
Conclusions:
As a quick and easy detection of Staphylococcus aureus, the LAMP technology was successfully established, laid the foundation for the diagnosis and treatment of children Staphylococcus aureus sepsis, and showed great promotion and application value.
Insights
Loop-mediated isothermal amplification (LAMP) offers a rapid and effective diagnostic method for neonatal sepsis. This technology successfully detects Staphylococcus aureus, improving diagnosis and treatment for pediatric sepsis.
Area of Science:
- Microbiology
- Molecular Diagnostics
- Neonatal Care
Background:
- Neonatal sepsis and bacterial meningitis require rapid and accurate diagnosis for effective treatment.
- Current diagnostic methods can be time-consuming, delaying critical interventions.
- Staphylococcus aureus is a significant pathogen in neonatal infections.
Purpose of the Study:
- To develop and evaluate a rapid diagnostic method for neonatal sepsis and bacterial meningitis.
- To establish a loop-mediated isothermal amplification (LAMP) assay for the specific detection of Staphylococcus aureus.
Main Methods:
- Primers were designed based on the 16S rRNA gene of Staphylococcus aureus.
- Loop-mediated isothermal amplification (LAMP) assay was used to test various bacterial specimens.
- 118 clinical sepsis and non-sepsis specimens were analyzed using both LAMP and blood culture.
Main Results:
- LAMP technology was successfully established for the specific detection of Staphylococcus aureus.
- The LAMP assay demonstrated a significantly higher detection rate compared to the traditional blood culture method (P <0.01).
Conclusions:
- LAMP technology provides a quick and easy method for detecting Staphylococcus aureus.
- This established LAMP assay serves as a foundation for diagnosing and treating pediatric Staphylococcus aureus sepsis.
- The technology holds significant potential for clinical application and promotion in healthcare settings.
