Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Effect of Aging on Tissues

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

MiR-27a promotes hepatocellular carcinoma cell proliferation through suppression of its target gene peroxisome proliferator-activated receptor γ.

Chinese medical journal·2015

Same author

Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1.

Applied biochemistry and biotechnology·2015

Same author

Herb-Partitioned Moxibustion and the miRNAs Related to Crohn's Disease: A Study Based on Rat Models.

Evidence-based complementary and alternative medicine : eCAM·2015

Same author

Bioactive carbazole alkaloids from the stems of Clausena lansium.

Fitoterapia·2015

Same author

Clauemarazoles A-G, seven carbazole alkaloids from the stems of Clausena emarginata.

Fitoterapia·2015

Same author

Scalable and DiI-compatible optical clearance of the mammalian brain.

Frontiers in neuroanatomy·2015

Search research articles

Related Experiment Videos

SIRT3 reverses aging-associated degeneration.

Katharine Brown¹, Stephanie Xie, Xiaolei Qiu

¹Program in Metabolic Biology, Nutritional Sciences & Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.

|February 5, 2013

Summary

Sirtuins, like SIRT3, are crucial for extending lifespan. Upregulating SIRT3 in aged hematopoietic stem cells (HSCs) can reverse age-related decline and restore their regenerative function.

Related Experiment Videos

Area of Science:

Aging and longevity research
Mitochondrial biology
Stem cell biology

Background:

Sirtuins are linked to lifespan extension, but their role in reversing aging is unclear.
Aging impairs stem cell function, affecting tissue repair and homeostasis.
SIRT3 regulates mitochondrial protein acetylation and oxidative stress.

Purpose of the Study:

To investigate the role of SIRT3 in aging-associated hematopoietic stem cell (HSC) dysfunction.
To determine if SIRT3 can reverse age-related degeneration in HSCs.
To explore the potential of targeting mitochondrial homeostasis for anti-aging interventions.

Main Methods:

Analysis of SIRT3 expression in hematopoietic stem cells (HSCs) across different ages.
Assessment of HSC function and regenerative capacity in young and aged mice with varying SIRT3 levels.
Mitochondrial protein acetylation and oxidative stress measurements in HSCs.

Main Results:

SIRT3 is highly expressed in HSCs and regulates their stress response.
SIRT3 is suppressed in aged HSCs, leading to impaired function.
Upregulating SIRT3 in aged HSCs significantly improves their regenerative capacity and reverses aging-associated decline.

Conclusions:

SIRT3 plays a critical role in maintaining HSC function during aging.
Mitochondrial homeostasis regulated by SIRT3 is key to stem cell maintenance and tissue repair.
Targeting SIRT3 offers a potential therapeutic strategy to reverse aging-associated stem cell degeneration.