Oligodeoxyribonucleotides derived from salmon sperm DNA: an alternative to defibrotide
Chang-Ye Hui1, Yan Guo, Xi Zhang
1Shenzhen Prevention and Treatment Center for Occupational Disease, Shenzhen, Guangdong 518001, China. hcy_sypu@hotmail.com
Abstract:
Defibrotide is a single-stranded nucleic acid polymer originally derived from porcine mucosa. Cheap salmon sperm DNA is commercially available and widely used in drug production. In this study, oligodeoxyribonucleotides were successfully obtained from the controlled depolymerization of salmon sperm DNA. The obtained product shared similar chemical and biological properties with defibrotide produced by Gentium SpA, Italy. It was also found that oligodeoxyribonucleotides derived from non-mammalian origins could also directly stimulate tissue plasminogen activator (t-PA) release from cultured human endothelial cells, and enhance fibrinolytic activity in the rabbit.
