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Updated: May 14, 2026

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
Published on: February 17, 2016
Hematopoietic progenitors express myelin basic protein and ensheath axons in Shiverer brain
James Goolsby1, Tapas Makar, Suhayl Dhib-Jalbut
1Department of Neurology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. JGoolsby@paragonbioservices.com
Abstract:
Oligodendroglia are cells of the central nervous system (CNS) that form myelin sheath, which insulates neuronal axons. Neuropathologies of the CNS include dysmyelination of axons in multiple sclerosis and CNS trauma. Cell replacement is a promising but largely untested therapy for dysmyelination. Shiverer mouse, a genetic mutant that does not synthesize full-length myelin basic protein (MBP), a critical prerequisite protein in CNS myelin sheath formation, provides an unequivocal model for determining the potential of stem cells to become oligodendroglia. We demonstrate that adult wild-type mouse bone marrow stem cells can express MBP and ensheath axons when transplanted into Shiverer brain.
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