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Related Experiment Video

Updated: May 14, 2026

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Published on: January 1, 2017

Risedronate increases osteoblastic differentiation and function through connexin43.

Hyung Min Jeong¹, Yun-Hey Jin, You-Hee Choi

¹College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, Republic of Korea.

Biochemical and Biophysical Research Communications

|February 5, 2013

Summary

This summary is machine-generated.

Risedronate, a bisphosphonate drug, promotes osteoblast differentiation and enhances bone formation. It positively regulates connexin43 (Cx43) gene transcription, suggesting a novel mechanism for its bone-protective effects.

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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14⁺ Monocytes

Published on: January 27, 2023

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Last Updated: May 14, 2026

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Published on: January 1, 2017

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14⁺ Monocytes

Published on: January 27, 2023

Area of Science:

Bone Biology and Pharmacology
Cellular and Molecular Medicine

Background:

Bisphosphonates are key antiresorptive drugs for osteoporosis, inhibiting osteoclast function.
Beyond antiresorption, bisphosphonates may promote osteoblast survival via anti-apoptotic effects mediated by connexin43 (Cx43) hemi-gap junctions.

Purpose of the Study:

To investigate the effects of risedronate on osteoblast differentiation.
To examine the impact of risedronate on connexin43 (Cx43) expression in mesenchymal cells.

Main Methods:

Utilized the C2C12 mesenchymal cell line.
Assessed osteoblast differentiation markers (OSE-luciferase, OC-luciferase, BSP-luciferase, ALP activity) and Cx43 promoter activity.
Analyzed protein expression of Cx43, Runx2, Osterix, and Dlx5.

Main Results:

Risedronate dose-dependently increased osterix (OSE) reporter activity.
Osteocalcin (OC) and bone sialoprotein (BSP) reporter activities were enhanced by risedronate.
Risedronate boosted alkaline phosphatase (ALP) activity, especially when combined with BMP2.
Risedronate increased Cx43 promoter activity and protein expression, alongside Runx2, Osterix, and Dlx5.

Conclusions:

Risedronate promotes osteoblastic differentiation in C2C12 cells.
Risedronate positively regulates Cx43 gene transcription and protein expression.
These findings suggest a role for risedronate in enhancing bone formation through osteoblast modulation and Cx43 regulation.