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Updated: May 14, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Solution structure of the WNK1 autoinhibitory domain, a WNK-specific PF2 domain
Thomas M Moon1, Fernando Correa, Lisa N Kinch
1Department of Pharmacology, College of Medicine, University of Vermont, 89 Beaumont Avenue, Burlington, VT 05405, USA. thomas.moon@uvm.edu
Abstract:
WNK1 [with no lysine (K)-1] is a 250-kDa serine/threonine protein kinase involved in the maintenance of cellular salt levels and is directly linked to a hereditary form of hypertension. Here, we report the solution NMR structure of the autoinhibitory domain of WNK1 (WNK1-AI), a small regulatory subunit that lies immediately C-terminal of the kinase domain. We show that this domain is a homolog of the RFXV-binding PASK/FRAY homology 2 (PF2) domain found in OSR (oxidative stress responsive) and SPAK (serine/threonine proline-alanine-rich) kinases, which are substrates of WNK1. The WNK1-AI has a circularly permuted topology relative to the OSR1-PF2 domain. Nevertheless, like PF2 domains, WNK1-AI binds peptides that contain an RFXV motif with micromolar affinities as assessed by changes in (1)H,(15)N heteronuclear single quantum coherence spectra. Mutations to the WNK1-AI and binding peptides confirm a similar binding mode.
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