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Updated: May 14, 2026

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

Stem cells: insights into the secretome.

Manousos Makridakis¹, Maria G Roubelakis, Antonia Vlahou

¹Biotechnology Division, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.

Biochimica Et Biophysica Acta

|February 5, 2013

Summary

Stem cell secretomes, the molecules they release, are crucial for tissue repair and regeneration. Research using proteomics reveals their therapeutic potential for various diseases.

Keywords:

2 Dimensional Differential Gel Electrophoresis 2 Dimensional Electrophoresis 2D-DIGE 2DE AF-MSCs AHF AT tau Acute Hepatic Failure Alzheimer's truncated tau Amniotic Fluid derived Mesenchymal Stem Cells BMSCs Bone Marrow derived Mesenchymal Stem Cells CKD CM CSCs Cancer Stem cells Chronic Kidney Disease Conditioned Medium Cyr 61 Cysteine rich protein 61 GRO GSCs GeLC-MS/MS Glioblastoma Stem like Cells Growth Related Oncogene HDGF HPL HUVEC Hepatic Progenitor-Like cells Hepatoma Derived Growth Factor Human Umbilical Vein Endothelial Cells IE-MS IFN γ IL-8 IP-10 Interferon gamma Interferon gamma-induced Protein 10 Interleukin 8 Iterative Exclusion-Mass Spectrometry Left-right determination factor 1 Lefty 1 MCP-1 MEF MIAMI MS Marrow Isolated Adult Multilineage Inducible cells Mass Spectrometry Monocyte Chemotactic Protein 1 Mouse Embryonic Fibroblast Murine Mesenchymal Stem Cells NSCs Neural Stem Cells OSM Osteogenic Medium Ovx PAI-1 Plasminogen Activator Inhibitor 1 Proteomics SDF-1 SDS PAGE combined with Liquid Chromatography Tandem Mass Spectrometry SMOC1 SPARC related Modular Calcium-binding protein 1 Secretome Stem cells Stromal cell-Derived Factor 1 TNF-α Tumor Necrosis Factor alpha VEGF Vascular Endothelial Growth Factor hAFSCs hAMSCs hASCs hBMSCs hESCs hMADS human Adipose tissue-derived Mesenchymal Stem Cells human Adipose tissue-derived Stem Cells human Amniotic Fluid Stem Cells human Bone Marrow-derived Mesenchymal Stem Cells human Embryonic Stem Cells human Multipotent Adipose-Derived Stem cells mESCs mMSCs murine Embryonic Stem Cells ovariectomized

Related Experiment Videos

Last Updated: May 14, 2026

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

Area of Science:

Biochemistry
Cell Biology
Regenerative Medicine

Background:

Stem cells are investigated as therapeutic agents for cardiovascular diseases, neurodegenerative disorders, and cancer.
The cell secretome, comprising secreted molecules, plays a vital role in cell-cell communication and tissue interactions.
Cellular functions like proliferation, differentiation, communication, and migration are regulated by the secretome.

Purpose of the Study:

To review key findings on stem cell secretomes.
To highlight the application of proteomics in studying stem cell secretomes.
To discuss the current research landscape in this field.

Main Methods:

Proteomics methods are employed to investigate the stem cell secretome.
Analysis of secreted molecules from stem cells.

Main Results:

The stem cell secretome contains crucial molecules that mediate cell-cell interactions and tissue crosstalk.
Proteomics has enabled detailed characterization of the stem cell secretome.
Research indicates significant potential for stem cell secretome applications in regenerative medicine.

Conclusions:

The stem cell secretome is a critical area of research with therapeutic implications.
Proteomics provides powerful tools for understanding stem cell secretome functions.
Continued investigation is essential for advancing regenerative medicine strategies.