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Stem cells: insights into the secretome

Manousos Makridakis1, Maria G Roubelakis, Antonia Vlahou

  • 1Biotechnology Division, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.

Biochimica Et Biophysica Acta
|February 5, 2013
PubMed

Insights

Stem cell secretomes, the molecules they release, are crucial for tissue repair and regeneration. Research using proteomics reveals their therapeutic potential for various diseases.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Regenerative Medicine

Background:

  • Stem cells are investigated as therapeutic agents for cardiovascular diseases, neurodegenerative disorders, and cancer.
  • The cell secretome, comprising secreted molecules, plays a vital role in cell-cell communication and tissue interactions.
  • Cellular functions like proliferation, differentiation, communication, and migration are regulated by the secretome.

Purpose of the Study:

  • To review key findings on stem cell secretomes.
  • To highlight the application of proteomics in studying stem cell secretomes.
  • To discuss the current research landscape in this field.

Main Methods:

  • Proteomics methods are employed to investigate the stem cell secretome.
  • Analysis of secreted molecules from stem cells.

Main Results:

  • The stem cell secretome contains crucial molecules that mediate cell-cell interactions and tissue crosstalk.
  • Proteomics has enabled detailed characterization of the stem cell secretome.
  • Research indicates significant potential for stem cell secretome applications in regenerative medicine.

Conclusions:

  • The stem cell secretome is a critical area of research with therapeutic implications.
  • Proteomics provides powerful tools for understanding stem cell secretome functions.
  • Continued investigation is essential for advancing regenerative medicine strategies.
Keywords:
2 Dimensional Differential Gel Electrophoresis2 Dimensional Electrophoresis2D-DIGE2DEAF-MSCsAHFAT tauAcute Hepatic FailureAlzheimer's truncated tauAmniotic Fluid derived Mesenchymal Stem CellsBMSCsBone Marrow derived Mesenchymal Stem CellsCKDCMCSCsCancer Stem cellsChronic Kidney DiseaseConditioned MediumCyr 61Cysteine rich protein 61GROGSCsGeLC-MS/MSGlioblastoma Stem like CellsGrowth Related OncogeneHDGFHPLHUVECHepatic Progenitor-Like cellsHepatoma Derived Growth FactorHuman Umbilical Vein Endothelial CellsIE-MSIFN γIL-8IP-10Interferon gammaInterferon gamma-induced Protein 10Interleukin 8Iterative Exclusion-Mass SpectrometryLeft-right determination factor 1Lefty 1MCP-1MEFMIAMIMSMarrow Isolated Adult Multilineage Inducible cellsMass SpectrometryMonocyte Chemotactic Protein 1Mouse Embryonic FibroblastMurine Mesenchymal Stem CellsNSCsNeural Stem CellsOSMOsteogenic MediumOvxPAI-1Plasminogen Activator Inhibitor 1ProteomicsSDF-1SDS PAGE combined with Liquid Chromatography Tandem Mass SpectrometrySMOC1SPARC related Modular Calcium-binding protein 1SecretomeStem cellsStromal cell-Derived Factor 1TNF-αTumor Necrosis Factor alphaVEGFVascular Endothelial Growth FactorhAFSCshAMSCshASCshBMSCshESCshMADShuman Adipose tissue-derived Mesenchymal Stem Cellshuman Adipose tissue-derived Stem Cellshuman Amniotic Fluid Stem Cellshuman Bone Marrow-derived Mesenchymal Stem Cellshuman Embryonic Stem Cellshuman Multipotent Adipose-Derived Stem cellsmESCsmMSCsmurine Embryonic Stem Cellsovariectomized

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