Related Experiment Video
Updated: May 14, 2026
06:57
Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos
Published on: July 21, 2021
Overexpression of PACEsol improves BMP-7 processing in recombinant CHO cells
Madhavi Sathyamurthy1, Jae Seong Lee, Jin Hyoung Park
1Department of Biological Sciences and Graduate School of Nanoscience & Technology, KAIST, Daejeon, Republic of Korea.
Journal of Biotechnology
|February 5, 2013
Summary
Overexpressing the paired basic amino acid cleaving enzyme (PACEsol) in CHO cells significantly increased mature Bone Morphogenetic Protein-7 (BMP-7) production by efficiently processing precursor forms.
Area of Science:
- Biotechnology
- Molecular Biology
- Cell Biology
Background:
- Bone morphogenetic protein-7 (BMP-7) is crucial for cartilage, bone, and kidney development.
- BMP-7 is synthesized as a precursor requiring proteolytic cleavage for activation.
- CHO cells producing recombinant human BMP-7 (rhBMP-7) secrete significant precursor forms.
Purpose of the Study:
- To enhance the production of mature BMP-7 from recombinant CHO cell cultures.
- To address the issue of insufficient proteolytic cleavage of BMP-7 precursor forms.
- To improve downstream purification by increasing the yield of active BMP-7.
Main Methods:
- Overexpression of a soluble form of paired basic amino acid cleaving enzyme (PACEsol) in CHO-BMP-7 cells.
- Cultivation of engineered CHO cells in serum-free suspension culture.
- Analysis of secreted rhBMP-7 forms in culture supernatants.
Main Results:
- Overexpression of PACEsol effectively processed BMP-7 precursor forms.
- Secreted rhBMP-7 in PACEsol-overexpressing cells consisted of nearly 100% mature form.
- Cell growth was not significantly affected by PACEsol overexpression.
Conclusions:
- PACEsol overexpression is an efficient strategy to boost mature BMP-7 production in CHO cells.
- This method simplifies downstream purification by eliminating precursor removal.
- Enhanced BMP-7 processing facilitates biotechnological applications of this growth factor.
