Accurate analysis of formose reaction products by LC-UV: an analytical challenge

This study presents a liquid chromatography-ultraviolet (LC-UV) method for the accurate analysis of formose reaction products with regard to sum parameters. Limitations encountered in gas chromatography (e.g., thermal stability of analytes) were overcome. First, alditols are O-benzoylated and subjected to reversed-phase chromatography. They are then detected by UV and by electrospray ionization-mass spectrometry (ESI-MS) for validation purposes. Compared with standard 2,4-dinitrophenylhydrazine labeling, the accuracy of results from LC-UV is dramatically improved due to enhanced chromatographic resolution and validation by ESI-MS. For the first time ever, LC-UV may be used to accurately quantify 2-hydroxymethylglycerol, a branched chain alditol, D,L-glyceraldehyde, D,L-galactose, which represents the group of D,L-iditol/galactose/glucose/mannose and D,L-erythrose in formose reaction products.