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Low oxygen concentrations for embryo culture in assisted reproductive technologies

E Mantikou1, S Bontekoe, M van Wely

  • 1Center for Reproductive Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Human Reproduction Update
|February 5, 2013
PubMed
Abstract

No abstract available in PubMed .

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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