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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 14, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

MicroRNA target prediction and validation.

William Ritchie¹, John E J Rasko, Stéphane Flamant

¹University of Sydney, Newtown, Australia. w.ritchie@centenary.usyd.edu.au

Advances in Experimental Medicine and Biology

|February 5, 2013

Summary

This summary is machine-generated.

Accurate microRNA target prediction is key to understanding microRNA function. This study reviews computational and experimental methods, highlighting the target reporter assay for validating microRNA-regulated genes.

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Last Updated: May 14, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression.
Accurate identification of miRNA targets is crucial for understanding miRNA function and biological roles.
Computational predictions suggest widespread miRNA regulation across the human genome.

Purpose of the Study:

To review and discuss computational and experimental methodologies for identifying mammalian microRNA targets.
To guide researchers in selecting appropriate methods for miRNA target prediction and validation.
To emphasize the importance of experimental validation for functional miRNA target sites.

Main Methods:

Review of computational miRNA target prediction algorithms and databases.
Discussion of experimental techniques for miRNA target identification.
Detailed explanation of the target reporter assay for validating functional miRNA-target interactions.

Main Results:

Computational methods offer various resources for predicting potential miRNA targets.
Experimental validation is essential to confirm computationally predicted targets.
The target reporter assay is presented as a reliable method for validating functional miRNA target sites.

Conclusions:

A combination of computational prediction and experimental validation is necessary for accurate miRNA target identification.
The target reporter assay provides specific and reliable validation of functional miRNA target sites.
Understanding miRNA targets is fundamental for deciphering gene regulation and cellular processes.