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Related Experiment Video

Updated: May 14, 2026

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

An optical fiber-based uncaging system.

Karl Kandler, Tuan Nguyen, Jihyun Noh

    Cold Spring Harbor Protocols
    |February 5, 2013
    PubMed
    Summary
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    Researchers developed an affordable ultraviolet-flash system for photolysis of caged compounds in neural tissues. This tool aids in stimulating neurons and mapping brain slice inputs effectively.

    Area of Science:

    • Neuroscience
    • Biophysics
    • Optical Imaging

    Background:

    • Photolysis of caged compounds is crucial for studying neuronal function.
    • Existing systems can be complex and costly.
    • Rapid and precise uncaging is needed for dynamic biological processes.

    Purpose of the Study:

    • To describe the assembly and performance of a novel, cost-effective ultraviolet-flash system.
    • To demonstrate its utility for focal photolysis in neural preparations.
    • To illustrate applications in neuronal stimulation and functional input mapping.

    Main Methods:

    • Assembly of a simple and inexpensive ultraviolet-flash system.
    • Application of the system for rapid focal photolysis of caged compounds.
    • Testing in cultured neurons and brain slices.

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    A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
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    A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

    Published on: January 7, 2019

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    Last Updated: May 14, 2026

    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
    10:29

    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

    Published on: October 8, 2014

    In vivo Optogenetic Stimulation of the Rodent Central Nervous System
    09:37

    In vivo Optogenetic Stimulation of the Rodent Central Nervous System

    Published on: January 15, 2015

    A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
    09:03

    A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

    Published on: January 7, 2019

  • Demonstration of neuronal stimulation and functional input mapping.

    • Main Results:

    • The system is suitable for rapid focal photolysis in cultured neurons and brain slices.
    • It offers advantages in simplicity and cost-effectiveness.
    • Performance characteristics and limitations were evaluated.
    • Successful application in stimulating neurons and mapping functional inputs was shown.

    Conclusions:

    • The developed ultraviolet-flash system provides an accessible tool for neuroscience research.
    • It enables precise uncaging of compounds for studying neuronal dynamics.
    • This system facilitates research on neuronal connectivity and function in brain slices.