An optical fiber-based uncaging system
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Updated: May 14, 2026
10:29
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
Published on: October 8, 2014
Cold Spring Harbor Protocols
|February 5, 2013
Abstract:
This article describes the assembly and performance of a simple and inexpensive ultraviolet-flash system suitable for rapid focal photolysis of caged compounds in cultured neurons and brain slices. Advantages and limitations of this system are discussed. Examples are provided illustrating how this system can be used for stimulating neurons and mapping their functional inputs in brain slices.
