Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

The Proteasome

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CAZyme fold architecture is conserved between disparate environments despite extreme sequence divergence.

mSystems·2026

Same author

Sharing big data for sustainable agri-food innovation.

Nature biotechnology·2026

Same author

Herd immunity in crops? Lessons from human epidemiology.

Trends in plant science·2026

Same author

Comparative transcriptomic profiling of field-grown cassava genotypes across season transitions.

Scientific data·2025

Same author

Transcriptomic responses of beet to infection by beet mild yellowing virus.

BMC plant biology·2025

Same author

Breeding for Integrated Pest Management (B-IPM): a new concept simultaneously optimising plant resistance and biocontrol.

Frontiers in plant science·2025

Same journal

Untargeted metabolomics reveals the metabolic basis of sugar-acid balance and quality differentiation in melon.

Frontiers in plant science·2026

Same journal

Biogenic volatile organic compound emission characteristics of dominant tree species in temperate broad-leaved Korean pine forests in Northeast China.

Frontiers in plant science·2026

Same journal

Study on differences in flavonoid synthesis in <i>Xanthoceras sorbifolia</i> leaves based on transcriptome analysis.

Frontiers in plant science·2026

Same journal

Evolutionary diversification of the <i>STAYGREEN</i> gene family in <i>Nicotiana</i>.

Frontiers in plant science·2026

Same journal

Identification and fungicide sensitivity of <i>Monosporascus lespedezae</i> sp. nov. causing root rot of <i>Lespedeza davurica</i> in Gansu, China.

Frontiers in plant science·2026

Same journal

Editorial: Plant phenotyping for agriculture.

Frontiers in plant science·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 14, 2026

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Plant secretome proteomics.

Erik Alexandersson¹, Ashfaq Ali, Svante Resjö

¹Department of Plant Protection Biology, Swedish University of Agricultural Sciences Alnarp, Sweden.

Frontiers in Plant Science

|February 5, 2013

Summary

This summary is machine-generated.

This review explores the plant secretome, focusing on methods to isolate and identify secreted proteins. It highlights key protein families and discusses alternative secretion pathways in plants.

Keywords:

apoplast mass spectrometry plant proteomics secretome

More Related Videos

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

Related Experiment Videos

Last Updated: May 14, 2026

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

Area of Science:

Plant biology
Molecular biology
Biochemistry

Background:

The plant secretome comprises proteins secreted into the apoplast, vital for cell structure, signaling, and stress responses.
While many secreted proteins have signal peptides, plants exhibit alternative secretion pathways for proteins lacking these.
Understanding the secretome is crucial for plant defense and development.

Purpose of the Study:

To review techniques for isolating and identifying plant secretomes.
To present bioinformatic tools for predicting secretion signals.
To highlight key protein families within the plant secretome.

Main Methods:

Proteomic studies for secretome analysis.
Bioinformatic tools for signal peptide prediction.
Literature review of existing research on plant secretomes.

Main Results:

Detailed overview of methods for plant secretome isolation and protein identification.
Introduction to bioinformatic approaches for defining the putative secretome.
Identification of significant protein families like proteases and hydrolases in plant secretomes.

Conclusions:

The plant secretome is complex, involving both classical and alternative secretion routes.
Advanced techniques and bioinformatic tools are essential for comprehensive secretome analysis.
Further research into the plant secretome will enhance understanding of plant-environment interactions.