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Updated: May 14, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

An automated system for high-throughput single cell-based breeding.

Nobuo Yoshimoto¹, Akiko Kida, Xu Jie

¹Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan. n-yosi44@agr.nagoya-u.ac.jp

Scientific Reports

|February 5, 2013

Summary

This summary is machine-generated.

This study introduces an automated single-cell analysis system for high-throughput screening and isolation of optimal cells. The system successfully identified superior embryonic stem cells and hybridomas, overcoming limitations of conventional methods.

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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Area of Science:

Biotechnology
Cell Biology
Regenerative Medicine

Background:

Cell heterogeneity in isogenic populations complicates selection for regenerative medicine and biopharmaceutical production.
Conventional high-throughput screening methods like fluorescence-activated cell sorting have limitations in refining clonal cell cultures.

Purpose of the Study:

To develop and demonstrate an automated, non-disruptive single-cell analysis and isolation system for high-throughput screening.
To establish superior cell lines for regenerative medicine and biopharmaceutical production by overcoming cell heterogeneity challenges.

Main Methods:

Employed an automated system for high-throughput, non-disruptive single-cell analysis and isolation.
Isolated and expanded single cells to establish clonal cell lines.
Utilized the system to screen for embryonic stem cells with high Rex1 expression and hybridomas with high antibody secretion.

Main Results:

Successfully established cell lines with desired characteristics, including embryonic stem cells exhibiting maximal pluripotency marker Rex1 expression.
Achieved high antibody secretion in hybridoma cell lines, surpassing capabilities of conventional screening systems.
Demonstrated the system's ability to select optimal cells that were previously unattainable.

Conclusions:

The developed single cell-based breeding system is a powerful tool for selecting superior cells from large libraries.
This technology enables the analysis of stochastic fluctuations and the elucidation of their underlying molecular mechanisms.
The system offers significant advantages over traditional high-throughput cell screening methods for various biotechnological applications.