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Related Experiment Video

Updated: May 14, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Transcriptome analysis of embryo maturation in maize.

Keat Thomas Teoh1, Deborah Vicuna Requesens, Shivakumar P Devaiah

  • 1Arkansas State University Biosciences Institute, State University, AR, USA.

BMC Plant Biology
|February 6, 2013
PubMed
Summary

This study analyzed gene expression during maize embryo development, identifying key genes for maturation and storage. A novel cupin gene shows promise for enhancing foreign protein expression in maize.

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Area of Science:

  • Plant Biology
  • Genomics
  • Biotechnology

Background:

  • Maize is a critical global crop requiring increased yield for food and feed.
  • Understanding maize embryo development is crucial for improving grain production.
  • Global gene expression analysis of maize embryos was previously limited.

Purpose of the Study:

  • To perform a comprehensive transcriptomic analysis of normal maize embryo maturation.
  • To identify genes vital for maize embryo development and storage reserve accumulation.
  • To explore potential new candidates for foreign gene expression in maize.

Main Methods:

  • Transcriptomic analysis of maize embryos at 15, 21, and 27 days after pollination (DAP).
  • Analysis of over 19,000 genes to identify key developmental and maturation genes.

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

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Last Updated: May 14, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

  • Quantitative reverse transcription PCR (qRT-PCR) to confirm gene expression levels.

    • Main Results:

    • Identified numerous genes with changing expression levels during embryo maturation (15-27 DAP).
    • Characterized gene expression in primary metabolic pathways, DNA synthesis, and storage proteins.
    • Discovered that over 30% of transcripts were un-annotated, indicating novel functions.

    Conclusions:

    • Identified novel candidate genes and promoters for maize embryo development and foreign protein expression.
    • Highlighted globulin-1, globulin-2, and a cupin family gene for their roles in storage.
    • The unidentified cupin family gene exhibits higher expression than globulin-1, suggesting its potential for enhanced foreign protein expression.