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An Experimental System to Study Mechanotransduction in Fetal Lung Cells
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An Experimental System to Study Mechanotransduction in Fetal Lung Cells

Published on: February 16, 2012

Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity

J R Mackenzie1, M Truesdale

  • 1Clinical Chemistry Laboratory, Methodist Hospital of Indiana, Indianapolis 46202.

Clinical Chemistry
|May 1, 1990
PubMed

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Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...