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Related Experiment Video

Updated: May 14, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Micro-volume wall-less immunoassays using patterned planar plates.

Katherine R Kozak¹, Jianyong Wang, Melvin Lye

¹Department of Biochemical and Cellular Pharmacology, Genentech, Inc., South San Francisco, CA, USA. kkozak@gene.com

|February 6, 2013

Summary

This summary is machine-generated.

This study introduces patterned planar plates (PPPs) for miniaturized immunoassays, significantly reducing sample and reagent volumes. PPPs offer comparable performance to traditional ELISAs while preventing cross-contamination and evaporation.

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Last Updated: May 14, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

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Published on: March 9, 2017

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Area of Science:

Biochemistry
Analytical Chemistry
Assay Development

Background:

Traditional enzyme-linked immunosorbent assays (ELISAs) face limitations in sample volume and potential for cross-contamination.
Miniaturization of immunoassays offers advantages in efficiency and reduced reagent consumption.

Purpose of the Study:

To present and evaluate a novel approach for miniaturized immunoassays using patterned planar plates (PPPs).
To compare the performance of immunoassays conducted on PPPs with traditional 384-well ELISA plates.

Main Methods:

Utilized 'wall-less' patterned planar plates with hydrophilic regions surrounded by hydrophobic polytetrafluoroethylene (PTFE).
Employed perfluorocarbon liquid (PFCL) overlay to prevent evaporation and wash buffer wetting during assays.
Performed and compared three immunoassays (FcRH5, SDF-1, human IgG) on PPPs versus 384-well ELISA plates.

Main Results:

PPP assays demonstrated comparable sensitivity, linearity, and quantitation to traditional ELISAs.
PPP assays required 10- to 50-fold less sample and reagent volume.
The PFCL overlay effectively prevented evaporation and inter-well cross-contamination.

Conclusions:

Patterned planar plates (PPPs) provide a viable and efficient method for miniaturizing plate-based immunochemical assays.
PPPs are compatible with existing equipment and workflows, offering a flexible alternative to traditional ELISAs.
This approach significantly reduces resource requirements while maintaining assay performance.