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Updated: May 14, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual
Igor Tkach1, Soraya Pornsuwan, Claudia Höbartner
1Research Group EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Abstract:
Pulsed electron-electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.
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